BS ISO 12078:2006
Current
The latest, up-to-date edition.
Anhydrous milk fat. Determination of sterol composition by gas liquid chromatography (Reference method)
Hardcopy , PDF
English
15-02-2006
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Foreword
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Sterol standard solutions
9.1.1 Calibrating solution for the determination
of cholesterol response factor
9.1.2 Qualitative solution for the determination
of sterol retention time
9.2 Test portion
9.3 Saponification
9.4 Extraction of the unsaponifiable matter
9.5 Thin-layer chromatography (TLC)
9.5.1 Preparation of TLC plates
9.5.2 Preparation of glass plate-developing tank
9.5.3 TLC of unsaponifiable matter
9.6 Preparation of silyl derivatives
9.7 Qualitative analysis
9.8 Quantitative analysis
9.8.1 Calculation of response factor
9.8.2 Determination of the test portion
9.8.3 Calculation and expression of results
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Examples of the gas-liquid
chromatographic analysis
Annex B (informative) Results of an interlaboratory test
Bibliography
Specifies a gas liquid chromatographic reference method for the determination of the sterol composition of anhydrous milk fat extracted from dairy products.
| Committee |
AW/5
|
| DevelopmentNote |
Supersedes 04/30116842 DC. (03/2006)
|
| DocumentType |
Standard
|
| Pages |
26
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
| Standards | Relationship |
| ISO 12078:2006 | Identical |
| ISO 5725-1:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions |
| ISO 707:2008 | Milk and milk products — Guidance on sampling |
| ISO 14156:2001 | Milk and milk products — Extraction methods for lipids and liposoluble compounds |
| ISO 6799:1991 | Animal and vegetable fats and oils Determination of composition of the sterol fraction Method using gas chromatography |
| ISO 5725-2:1994 | Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
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