BS ISO 13641-2:2003
Current
The latest, up-to-date edition.
Water quality. Determination of inhibition of gas production of anaerobic bacteria Test for low biomass concentrations
Hardcopy , PDF
English
31-03-2014
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Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents and media
5 Apparatus
6 Test environment and interferences
7 Procedure
8 Calculation
9 Validity criteria
10 Test report
Annex A (informative) - Calibration of the pressure meter
Annex B (informative) - Expression of results in tests with
wastewater
Bibliography
Defines a screening method for assessing the potential toxicity of substances, mixtures, surface waters, groundwaters and wastewaters, effluents, sludges, or other environmental samples by determining the production of biogas (carbon dioxide and methane) from muds, sediments and other anaerobic environments with low biomass concentration.
| Committee |
EH/3/5
|
| DevelopmentNote |
Issued Date: 31/03/2014. Supersedes 99/566932 DC. (03/2014)
|
| DocumentType |
Standard
|
| Pages |
22
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This part of ISO13641 specifies a screening method for assessing the potential toxicity of substances, mixtures, surface waters, groundwaters and wastewaters, effluents, sludges, or other environmental samples by determining the production of biogas (carbon dioxide and methane) from muds, sediments and other anaerobic environments with low biomass concentration. The growth rate of anaerobic bacteria is much lower, compared with that of aerobic microorganisms. For this reason, the test periods in anaerobic methods are longer than in those with aerobic bacteria. The conditions of this test (for example amount of inoculum and substrate in the test bottles) were adopted to a defined test period over several days. The inoculum can be collected from anaerobic sediments or from large, or laboratory scale, anaerobic digesters.
This method is applicable to materials, soluble or insoluble in water, including volatile chemicals (see Reference [1] in the Bibliography).
NOTE Special care is necessary with compounds of low water-solubility, and in these cases, see for example, ISO10634. For general information on biotesting see ISO5667-16[2].
Information obtained by this method can be helpful prior to anaerobic biodegradability testing with low inoculum mass concentrations and for estimations of the potential effects of chemicals and wastewater to anaerobic processes in habitats characterized by a relatively low anaerobic biomass, for example natural sediments and soils.
| Standards | Relationship |
| ISO 13641-2:2003 | Identical |
| ISO 11923:1997 | Water quality — Determination of suspended solids by filtration through glass-fibre filters |
| ISO 11734:1995 | Water quality — Evaluation of the "ultimate" anaerobic biodegradability of organic compounds in digested sludge — Method by measurement of the biogas production |
| ISO 5667-16:2017 | Water quality — Sampling — Part 16: Guidance on biotesting of samples |
| ISO 10634:1995 | Water quality Guidance for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in an aqueous medium |
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