BS ISO 14502-2:2005
Current
The latest, up-to-date edition.
Determination of substances characteristic of green and black tea Content of catechins in green tea. Method using high-performance liquid chromatography
Hardcopy , PDF
English
31-07-2006
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Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparation of test samples
8 Procedure
8.1 General
8.2 Determination of dry matter content
8.3 Test portion
8.4 Extraction of polyphenols
8.5 Dilution
8.6 Determination
9 Calculation
9.1 General
9.2 Quantitation using catechin standards
9.3 Quantitation using catechin standard and catechin
Relative Response Factors
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Results of interlaboratory tests
Annex B (informative) Assessment of purity of standards
Annex C (informative) Typical HPCL chromatograms
Annex D (informative) The effect of ferric ions on catechin RRFs
Annex E (informative) Quantitative comparison - Use of catechin
standards or a caffeine standard in conjunction
with catechin RRFs
Bibliography
Defines a high-performance liquid chromatographic (HPLC) method for the determination of the total catechin content of tea from the summation of the individual catechins. It is applicable to both leaf and instant green tea, and with precision limitations to black tea.
| Committee |
AW/8
|
| DevelopmentNote |
Supersedes 03/312777 DC. (04/2005)
|
| DocumentType |
Standard
|
| Pages |
30
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
| Standards | Relationship |
| ISO 14502-2:2005 | Identical |
| ISO 7516:1984 | Instant tea in solid form Sampling |
| ISO 1839:1980 | Tea — Sampling |
| ISO 7513:1990 | Instant tea in solid form Determination of moisture content (loss in mass at 103 degrees C) |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| ISO 1572:1980 | Tea Preparation of ground sample of known dry matter content |
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