BS ISO 14502-2:2005
Current
The latest, up-to-date edition.
Determination of substances characteristic of green and black tea Content of catechins in green tea. Method using high-performance liquid chromatography
Hardcopy , PDF
English
31-07-2006
Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparation of test samples
8 Procedure
8.1 General
8.2 Determination of dry matter content
8.3 Test portion
8.4 Extraction of polyphenols
8.5 Dilution
8.6 Determination
9 Calculation
9.1 General
9.2 Quantitation using catechin standards
9.3 Quantitation using catechin standard and catechin
Relative Response Factors
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Results of interlaboratory tests
Annex B (informative) Assessment of purity of standards
Annex C (informative) Typical HPCL chromatograms
Annex D (informative) The effect of ferric ions on catechin RRFs
Annex E (informative) Quantitative comparison - Use of catechin
standards or a caffeine standard in conjunction
with catechin RRFs
Bibliography
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