BS ISO 16212 : 2008
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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COSMETICS - MICROBIOLOGY - ENUMERATION OF YEAST AND MOULD
Hardcopy , PDF
31-07-2011
English
01-01-2008
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principles
4.1 General
4.2 Plate count
4.3 Membrane filtration
5 Diluents, neutralizers and culture media
5.1 General
5.2 Neutralizing diluents and diluents
5.3 Diluent for yeast suspension (tryptone sodium chloride
solution)
5.4 Culture media
6 Apparatus and glassware
7 Strain of microorganisms
8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendation
9.2 Preparation of the initial suspension
9.3 Counting methods
10 Counting of colonies (plate counts and membrane filtration
methods)
11 Expression of results
11.1 Method of calculation for plate count
11.2 Interpretation
12 Neutralization of the antifungicidal properties of the product
12.1 General
12.2 Preparation of inoculum
12.3 Validation of counting methods
13 Test report
Annex A (informative) - Other neutralizing diluents
Annex B (informative) - Other diluents
Annex C (informative) - Other culture media
Annex D (informative) - Neutralizers of antifungicidal activity of
preservatives and rinsing liquids
Bibliography
Provides general guidelines for enumeration of yeast and mould present in cosmetics by counting the colonies on selective agar medium after aerobic incubation.
| Committee |
CW/217
|
| DevelopmentNote |
Supersedes 07/30136068 DC. (10/2008) Renumbered & superseded by BS EN ISO 16212. (07/2011)
|
| DocumentType |
Standard
|
| Pages |
28
|
| PublisherName |
British Standards Institution
|
| Status |
Superseded
|
| SupersededBy | |
| Supersedes |
| Standards | Relationship |
| ISO 16212:2017 | Identical |
| EN 13624:2013 | Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity in the medical area - Test method and requirements (phase 2, step 1) |
| EN 12353:2013 | Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity |
| ISO 18415:2017 | Cosmetics — Microbiology — Detection of specified and non-specified microorganisms |
| ISO 18416:2015 | Cosmetics Microbiology Detection of Candida albicans |
| ISO 21148:2017 | Cosmetics — Microbiology — General instructions for microbiological examination |
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