BS ISO 21149 : 2006

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 General
   4.2 Plate count
   4.3 Membrane filtration
   4.4 Detection of bacteria by enrichment
5 Diluents, neutralizers and culture media
   5.1 General
   5.2 Neutralizing diluents and diluents
   5.3 Diluent for the bacterial suspension (tryptone sodium
        chloride solution)
   5.4 Culture media
6 Apparatus and glassware
7 Strains of microorganisms
8 Handling of cosmetic products and laboratory samples
9 Procedure
   9.1 General recommendation
   9.2 Preparation of the initial suspension
   9.3 Counting methods
   9.4 Enrichment
10 Counting of colonies (plate counts and membrane filtration
   methods)
11 Detection of growth (enrichment method)
12 Expression of results
   12.1 Method of calculation for plate count
   12.2 Interpretation
   12.3 Examples
   12.4 Detection after enrichment
13 Neutralization of the antimicrobial properties of the product
   13.1 General
   13.2 Preparation of inoculum
   13.3 Validation of counting methods
   13.4 Validation of the detection method by enrichment
   13.5 Interpretation of validation results
14 Test report
Annex A (informative) Other neutralizing diluents
Annex B (informative) Other diluents
Annex C (informative) Other culture media
Annex D (informative) Neutralizers of antimicrobial activity of
                      preservatives and rinsing liquids
Bibliography

Provides general guidelines for enumeration and detection of mesophilic aerobic bacteria present in cosmetics: - by counting the colonies on agar medium after aerobic incubation, or - by checking the absence of bacterial growth after enrichment.