BS ISO 21427-1:2006

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents
7 Apparatus
8 Treatment and preparation of samples
   8.1 Waters, effluents, leachates and eluates to be tested
   8.2 Substances to be tested
9 Procedure
   9.1 Selection of concentrations
   9.2 Conducting the test
   9.3 Reading of the histological preparations
10 Expression of results
   10.1 Presentation of results
   10.2 Processing of results
   10.3 Interpretation of results
11 Validity of the test
12 Test report
Annex A (normative) Data specific to Pleurodeles waltl
Annex B (normative) Breeding
Annex C (informative) Example of a statistical method
        for processing results
Bibliography

Describes a method for assessing genotoxicity using Amphibia larvae (Xenopus laevis and Pleurodeles waltl).

This part of ISO21427 specifies a method for assessing genotoxicity using Amphibia larvae (Xenopus laevis and Pleurodeles waltl). The provisions specific to Pleurodeles are described in AnnexA.

The method is applicable to:

• aqueous effluents;

• aqueous leachates;

• eluates of soils;

• eluates of industrial waste;

• eluates of sludges from sewage treatment;

• surface and ground water;

• water-soluble substances.