BS ISO 23443:2020

This document specifies a method for the quantitative determination of β-carotene and lycopene in infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) and UV-visible detection.

This document specifies a method for the quantitative determination of ?-carotene and lycopene in infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) and UV-visible detection. The application range runs from 1?g/100g to 1500?g/100g for lycopene and from 1?g/100g to 2250?g/100g for ?-carotene. Based on the single-laboratory validation, the limit of detection (LOD) was 0,1?g/100g and the limit of quantification (LOQ) was 0,3?g/100g for each carotenoid.The method does not apply to materials that contain measurable levels of ?-apo-8?-carotenal. The reproducibility data meets the requirements given in References[8] and [10].AnnexC specifies the determination of lutein. The reproducibility data does not meet the requirements given in Reference[9].