CLSI I/LA29 A : 1ED 2008

Abstract
Committee Membership
Foreword
1 Scope
2 Standard Precautions
3 Terminology
   3.1 A Note on Terminology
   3.2 Definitions
   3.3 Abbreviations/Acronyms
4 Biosafety
   4.1 Specimen Collection
   4.2 Safety Attire
   4.3 Biological Safety Cabinets
   4.4 Specimen Containers
   4.5 Centrifugation
   4.6 Pipetting
   4.7 Sharp Devices
   4.8 Blood Spills
   4.9 Waste Disposal and Specimen Inactivation
   4.10 Unfixed Specimens
   4.11 Equipment Disinfection
5 Solid Phase Methods
   5.1 Overview
   5.2 Specimens
   5.3 Attachment of Antigens to the Matrix
   5.4 Limitations for All Solid-Phase Assays
   5.5 Enzyme-Linked Immunosorbent Assay
   5.6 Microsphere-Based Multiplexing Flow System
   5.7 Microchips
6 Human Leukocyte Antigen Antibody Screening Methodologies
   6.1 Introduction
   6.2 Data Analysis and Interpretation of Solid Phase
        Human Leukocyte Antigen Antibody Screening Assays
   6.3 Quality Control for Solid Phase Antibody Screening Assays
   6.4 Limitations of Solid Phase Antibody Screening Assays
7 Identification Using Multiantigen Panels
   7.1 Introduction/Overview
   7.2 Data Analysis and Interpretation of Antibody Specificity
   7.3 Limitations of Multiantigen Methods and Technical
        Considerations
8 Single Antigen Approaches
   8.1 Introduction/Overview
   8.2 Data Analysis and Interpretation of Single Antigen
        (High Definition) Assays
   8.3 Quality Control for Solid Phase Single Antigen Assays
        for Human Leukocyte Antigen Antibody Specificity
   8.4 Limitations of Solid Phase Single Antigen Assays for
        Human Leukocyte Antigen Antibody Specificity
9 Flow Cytometric Crossmatch
   9.1 Background
   9.2 General Methodological Choices
   9.3 Basic Procedure
   9.4 Interpretation of Results
   9.5 Limitations/Problems
10 Clinical Relevance of Antibodies Identified Using Solid
   Phase Assays
   10.1 Background
   10.2 Clinical Relevance
   10.3 Advantages of Solid Phase Assays
   10.4 Limitations of Solid Phase Assays
   10.5 Uses for Solid Phase Assays
   10.6 Best Practices for Use of Solid Phase Assays
References
Appendix - Assessment of Delayed Shipping and Processing on
           Human Leukocyte Antigen Antibody
Results
Summary of Comments and Subcommittee Responses
The Quality Management System Approach
Related CLSI Reference Materials

Specifies criteria for optimizing methods that utilize flow cytometry and other conventional and multiplex platforms.