CLSI M11 A7 : 7ED 2007

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Indications for Performing Susceptibility Tests on
   Anaerobic Bacteria
   5.1 Routine Testing
   5.2 Surveillance Testing
6 Limitations of Susceptibility Testing of Anaerobic Bacteria
   6.1 Interpretive Criteria (Breakpoints) and Clinical
        Correlation
   6.2 Organisms
7 Antimicrobial Agents
   7.1 Source
   7.2 Weighing Antimicrobial Powders
   7.3 Preparing Stock Solutions
   7.4 Number of Concentrations Tested
8 Selection of Antimicrobial Agents for Routine Testing and
   Reporting
   8.1 Routine Reports
   8.2 Nonproprietary Names
9 Storage and Recovery of Isolates for Testing
   9.1 Freezing Isolates for Later Testing
   9.2 Recovering Isolates From Frozen Storage
10 Inoculum Preparation for Dilution Tests
   10.1 Turbidity Standard for Inoculum Preparation
   10.2 Growth Method
   10.3 Direct Colony Suspension Method
11 Beta-lactamase Testing
12 Reference Agar Dilution Procedure (Wadsworth Method)
   12.1 Reagents and Materials
   12.2 Procedure for Preparing Agar Dilution Plates
   12.3 Inoculation of Agar Dilution Plates
   12.4 Incubation of Agar Dilution Plates
   12.5 Reading Agar Dilution Plates
13 Broth Microdilution Procedure
   13.1 Reagents and Materials
   13.2 Procedure for Preparing Broth Microdilution Plates
   13.3 Inoculation of Broth Microdilution Trays
   13.4 Incubation of Broth Microdilution Trays
   13.5 Reading Broth Microdilution Endpoints
14 Quality Control Guidelines
   14.1 Purpose
   14.2 Control Organisms and Frequency of Testing
   14.3 Corrective Action
References
Appendix A. Preparation of Media and Supplements
Appendix B. Media, Reagents, Supplies, Equipment, and Timetable
            for Agar Dilution Susceptibility Testing of Anaerobic
            Bacteria

Provides reference methods for the determination of minimal inhibitory concentrations (MICs) of anaerobic bacteria by agar dilution and broth microdilution.