CLSI M2 A9 : 9ED 2006

Abstract
Committee Membership
Foreword
Subcommittee on Antimicrobial Susceptibility
Testing Mission Statement
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Indications for Performing Susceptibility Tests
6 Selection of Antimicrobial Agents for Routine
   Testing and Reporting
   6.1 Routine Reports
   6.2 Nonproprietary Names
   6.3 Selection Guidelines
   6.4 Suggested Guidelines for Routine and Selective
        Testing and Reporting
7 Reagents for the Disk Diffusion Test
   7.1 Mueller-Hinton Agar
   7.2 Storage of Antimicrobial Disks
8 Inoculum Preparation for Disk Diffusion Tests
   8.1 Turbidity Standard for Inoculum Preparation
   8.2 Inoculum Preparation
9 Procedure for Performing the Disk Diffusion Test
   9.1 Inoculation of Test Plates
   9.2 Application of Disks to Inoculated Agar Plates
   9.3 Reading Plates and Interpreting Results
10 Fastidious Organisms
   10.1 Haemophilus influenzae and H. parainfluenzae
   10.2 Neisseria gonorrhoeae
   10.3 Neisseria meningitidis
   10.4 Streptococcus pneumoniae and Other
        Streptococcus spp
11 Problem Organisms
   11.1 Staphylococci
   11.2 Enterococci
   11.3 Extended-Spectrum, a-Lactamase-Producing,
        Gram-Negative Bacilli
12 Inducible Clindamycin Resistance
13 a-Lactamase Tests
   13.1 Purpose
   13.2 Selecting a a-Lactamase Test
14 Interpretation of Disk Diffusion Test Results
   14.1 Zone Diameter Interpretive Standards
   14.2 Interpretive Categories
   14.3 Equivalent MIC Breakpoints
15 Quality Control Procedures
   15.1 Purpose
   15.2 Reference Strains for Quality Control
   15.3 Storing and Testing Quality Control Strains
   15.4 Zone Diameter Quality Control Limits
   15.5 Frequency of Quality Control Testing
   15.6 Corrective Action
   15.7 Reporting Patient Results When Out-of-Control
        Tests Occur
   15.8 Verification of Patient Test Results
16 Limitations of Disk Diffusion Methods
   16.1 Application to Various Organism Groups
   16.2 Misleading Results
   16.3 Emergence of Resistance
17 Screening Tests
References
Appendix A - Quality Control Protocol Flow Charts
Summary of Comments and Subcommittee Responses
The Quality System Approach
Related CLSI/NCCLS Publications

Defines the standard agar disk diffusion techniques to be utilized to determine the in vitro susceptibility of bacteria that grow aerobically.