CLSI M2 A9 : 9ED 2006
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTS
30-12-2008
12-01-2013
Abstract
Committee Membership
Foreword
Subcommittee on Antimicrobial Susceptibility
Testing Mission Statement
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Indications for Performing Susceptibility Tests
6 Selection of Antimicrobial Agents for Routine
Testing and Reporting
6.1 Routine Reports
6.2 Nonproprietary Names
6.3 Selection Guidelines
6.4 Suggested Guidelines for Routine and Selective
Testing and Reporting
7 Reagents for the Disk Diffusion Test
7.1 Mueller-Hinton Agar
7.2 Storage of Antimicrobial Disks
8 Inoculum Preparation for Disk Diffusion Tests
8.1 Turbidity Standard for Inoculum Preparation
8.2 Inoculum Preparation
9 Procedure for Performing the Disk Diffusion Test
9.1 Inoculation of Test Plates
9.2 Application of Disks to Inoculated Agar Plates
9.3 Reading Plates and Interpreting Results
10 Fastidious Organisms
10.1 Haemophilus influenzae and H. parainfluenzae
10.2 Neisseria gonorrhoeae
10.3 Neisseria meningitidis
10.4 Streptococcus pneumoniae and Other
Streptococcus spp
11 Problem Organisms
11.1 Staphylococci
11.2 Enterococci
11.3 Extended-Spectrum, a-Lactamase-Producing,
Gram-Negative Bacilli
12 Inducible Clindamycin Resistance
13 a-Lactamase Tests
13.1 Purpose
13.2 Selecting a a-Lactamase Test
14 Interpretation of Disk Diffusion Test Results
14.1 Zone Diameter Interpretive Standards
14.2 Interpretive Categories
14.3 Equivalent MIC Breakpoints
15 Quality Control Procedures
15.1 Purpose
15.2 Reference Strains for Quality Control
15.3 Storing and Testing Quality Control Strains
15.4 Zone Diameter Quality Control Limits
15.5 Frequency of Quality Control Testing
15.6 Corrective Action
15.7 Reporting Patient Results When Out-of-Control
Tests Occur
15.8 Verification of Patient Test Results
16 Limitations of Disk Diffusion Methods
16.1 Application to Various Organism Groups
16.2 Misleading Results
16.3 Emergence of Resistance
17 Screening Tests
References
Appendix A - Quality Control Protocol Flow Charts
Summary of Comments and Subcommittee Responses
The Quality System Approach
Related CLSI/NCCLS Publications
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