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CLSI M2 A9 : 9ED 2006

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTS

Superseded date

30-12-2008

Published date

12-01-2013

Abstract
Committee Membership
Foreword
Subcommittee on Antimicrobial Susceptibility
Testing Mission Statement
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Indications for Performing Susceptibility Tests
6 Selection of Antimicrobial Agents for Routine
   Testing and Reporting
   6.1 Routine Reports
   6.2 Nonproprietary Names
   6.3 Selection Guidelines
   6.4 Suggested Guidelines for Routine and Selective
        Testing and Reporting
7 Reagents for the Disk Diffusion Test
   7.1 Mueller-Hinton Agar
   7.2 Storage of Antimicrobial Disks
8 Inoculum Preparation for Disk Diffusion Tests
   8.1 Turbidity Standard for Inoculum Preparation
   8.2 Inoculum Preparation
9 Procedure for Performing the Disk Diffusion Test
   9.1 Inoculation of Test Plates
   9.2 Application of Disks to Inoculated Agar Plates
   9.3 Reading Plates and Interpreting Results
10 Fastidious Organisms
   10.1 Haemophilus influenzae and H. parainfluenzae
   10.2 Neisseria gonorrhoeae
   10.3 Neisseria meningitidis
   10.4 Streptococcus pneumoniae and Other
        Streptococcus spp
11 Problem Organisms
   11.1 Staphylococci
   11.2 Enterococci
   11.3 Extended-Spectrum, a-Lactamase-Producing,
        Gram-Negative Bacilli
12 Inducible Clindamycin Resistance
13 a-Lactamase Tests
   13.1 Purpose
   13.2 Selecting a a-Lactamase Test
14 Interpretation of Disk Diffusion Test Results
   14.1 Zone Diameter Interpretive Standards
   14.2 Interpretive Categories
   14.3 Equivalent MIC Breakpoints
15 Quality Control Procedures
   15.1 Purpose
   15.2 Reference Strains for Quality Control
   15.3 Storing and Testing Quality Control Strains
   15.4 Zone Diameter Quality Control Limits
   15.5 Frequency of Quality Control Testing
   15.6 Corrective Action
   15.7 Reporting Patient Results When Out-of-Control
        Tests Occur
   15.8 Verification of Patient Test Results
16 Limitations of Disk Diffusion Methods
   16.1 Application to Various Organism Groups
   16.2 Misleading Results
   16.3 Emergence of Resistance
17 Screening Tests
References
Appendix A - Quality Control Protocol Flow Charts
Summary of Comments and Subcommittee Responses
The Quality System Approach
Related CLSI/NCCLS Publications

Defines the standard agar disk diffusion techniques to be utilized to determine the in vitro susceptibility of bacteria that grow aerobically.

DevelopmentNote
Supersedes NCCLS M2 A8 Not available separately, Included in packages of: NCCLS M2 A9 and NCCLS M100 S16 NCCLS M2 A9, NCCLS M7 A7 and NCCLS M100 S16 (01/2006)
DocumentType
Miscellaneous Product
PublisherName
Clinical Laboratory Standards Institute
Status
Superseded
SupersededBy
Supersedes

CLSI M38 A2 : 2ED 2008 REFERENCE METHOD FOR BROTH DILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF FILAMENTOUS FUNGI
CLSI VET04 A : 1ED 2006 METHODS FOR BROTH DILUTION SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS
CLSI M35 A2 : 2ED 2008 ABBREVIATED IDENTIFICATION OF BACTERIA AND YEAST
CLSI M100 S16 : 16ED 2006 PERFORMANCE STANDARDS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING
CLSI M45 A : 1ED 2006 METHODS FOR ANTIMICROBIAL DILUTION AND DISK SUSCEPTIBILITY TESTING OF INFREQUENTLY ISOLATED OR FASTIDIOUS BACTERIA
CLSI VET03 A : 1ED 2006 METHODS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS
CLSI M7 A7 : 7ED 2006 METHODS FOR DILUTION ANTIMICROBIAL SUSCEPTIBILITY TESTS FOR BACTERIA THAT GROW AEROBICALLY
CLSI M27 S3 : 3ED 2008 REFERENCE METHOD FOR BROTH DILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEASTS
CLSI M23 A3 : 3ED 2008 DEVELOPMENT OF IN VITRO SUSCEPTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS
CLSI M27 A3 : 3ED 2008 REFERENCE METHOD FOR BROTH DILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEASTS
CLSI VET02 A3 : 3ED 2008 DEVELOPMENT OF IN VITRO SUSCEPTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS FOR VETERINARY ANTIMICROBIAL AGENTS
CLSI M31 A3 : 3ED 2008 PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK AND DILUTION SUSCEPTIBILITY TESTS FOR BACTERIA ISOLATED FROM ANIMALS
CLSI M50 P : 1ED 2008 QUALITY CONTROL FOR COMMERCIAL MICROBIAL IDENTIFICATION SYSTEMS

CLSI M23 A2 : 2ED 2001 DEVELOPMENT OF IN VITRO SUSPECTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS
CLSI GP2 A4 : 4ED 2002 CLINICAL LABORATORY TECHNICAL PROCEDURE MANUALS
CLSI M29 A3 : 3ED 2005 ACQUIRED INFECTIONS; APPROVED GUIDELINE
CLSI M45 P : 1ED 2005 METHODS FOR ANTIMICROBIAL DILUTION AND DISK SUSCEPTIBILITY TESTING OF INFREQUENTLY ISOLATED OR FASTIDIOUS BACTERIA
CLSI M11 A6 : 6ED 2004 METHODS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF ANAEROBIC BACTERIA
CLSI M39 A2 : 2ED 2005 ANALYSIS AND PRESENTATION OF CUMULATIVE ANTIMICROBIAL SUSCEPTIBILITY TEST DATA

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