CLSI M24 A : 1ED 2003

Abstract
Committee Membership
Foreword
1 Scope
2 Definitions
3 Antimycobacterial Susceptibility Testing for
   Mycobacterium tuberculosis Complex
   3.1 Introduction
   3.2 Agar Proportion Method
   3.3 Commercial Broth Systems with Shorter Incubation
       Time for Susceptibility Testing of MTBC
   3.4 PZA Susceptibility Testing
   3.5 Quality Control (QC)
   3.6 Implementing New methods
4 Susceptibility Testing of Slowly Growing Nontuberculous
   Mycobacteria
   4.1 Introduction
   4.2 Susceptibility Testing of Mycobacterium avium
       Complex (MAC)
   4.3 Susceptibility Testing of Mycobacterium kansasii
   4.4 Susceptibility Testing of Mycobacterium marinum
   4.5 Susceptibility Testing of Miscellaneous Slowly
       Growing Nontuberculous Mycobacteria
5 Antimycobacterial Susceptibility Testing for Rapidly
   Growing Mycobacteria
   5.1 Introduction
   5.2 Indications for Performing Susceptibility Tests
   5.3 Antimicrobial Agents and Concentrations
   5.4 Preparation of the Inoculum
   5.5 Susceptibility Test Method
   5.6 Report of MIC Results
   5.7 Quality Control
6 Susceptibility Testing of Aerobic Actinomycetes
   6.1 Method
   6.2 Antimicrobial Agents
   6.3 Reporting of Results
   6.4 Quality Control
Table 1 Antituberculous Drugs and Their Recommended
        Concentrations in Middlebrook 7H10 and 7H11
        Agar Medium
Table 2 Stock, Working, and Final Concentrations of
        Antituberculous Drug Solutions for Middlebrook
        7H10 Agar Medium
Table 3 Agar Proportion Method Using 7H10 Agar and
        Drug-Containing Disks for Susceptibility Testing
        of Mycobacterium tuberculosis
Table 4 Dilution of Sputum Concentrate for Inoculation of
        the Susceptibility Test Medium for the Direct
        Susceptibility Test
Table 5 Macrolide Interpretative Criteria for Mycobacterium
        avium Complex
Table 6 Proposed Antimycobacterial Agents, MIC Values
        Indicating Resistance, and Media for Testing of
        M. kansasii as well as Selected References Used to
        Evaluated the Test Concentrations
Table 7 Proposed Antimycobacterial Agents, MIC Values
        Indicating Resistance, and Media for
        Susceptibility Testing of Mycobacterium marinum
Table 8 Broth Microdilution Interpretive Criteria for
        Rapidly Growing Mycobacteria
Table 9 Tentative Quality Control Ranges of Minimal
        Inhibitory Concentrations (Microgram/mL) for
        Mycobacterium peregrinum ATCC(R) 700686 and
        Staphylococcus aureus ATCC(R) 29213
Table 10 Broth Microdilution Breakpoints for Nocardia and
         Other Aerobic Actinomycetes
References
Appendix A Case Scenarios for MTBC Drug Susceptibility
           Testing
Appendix B McFarland 0.5 Barium Sulfate Turbidity
           Standard
Appendix C Scenarios and Examples of Possible Reports
Appendix D Drugs Available for Susceptibility Testing
           of MTBC Using Commercial Shorter Incubation
           Systems Cleared for Use by the U.S. FDA and
           Their Equivalence in the Agar Proportion
           Method
Appendix E Procedure for Alkalization of Radiometric 12B
           Medium
Appendix F Agar Disk Elution Method for Testing Slowly
           Growing Nontuberculous Mycobacteria
Summary of Comments and Subcommittee Responses
Delegate Comment and Subcommittee Response
The Quality System Approach
Related NCCLS Publications