CLSI M41 P : 1ED 2006

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Monolayered Cell Cultures and Cell Culture Medium
  (Preanalytical)
  5.1 Cell Culture Variables Affecting Susceptibility
      to Viral Infection
  5.2 Types of Culture Vessels
  5.3 Assessment, Maintenance, and Quality Control of
      Uninoculated Monolayered Cell Cultures
  5.4 Cell Culture Medium
6 Specimens for Viral Culture (Preanalytical)
  6.1 General Principles
  6.2 Specimen Collection
  6.3 Transport and Storage
  6.4 Assessment Criteria
  6.5 Processing[20,21]
  6.6 Storage of Processed and Residual Specimens
7 Viral Culture Using Monolayered Cell Cultures
  (Analytical)
  7.1 Design of Viral Culture Algorithms
  7.2 Inoculation Procedure
  7.3 Incubation and Maintenance of Inoculated Cell Cultures
  7.4 Examination of Inoculated Cell Culture Monolayers
  7.5 Storage of Viral Isolates
8 Quality Assessment
  8.1 Summary of QI/QC Practices in This Document
  8.2 Troubleshooting
9 Postanalytical Considerations
  9.1 Levels of Identification
  9.2 Reporting Results
  9.3 Limitations of Viral Culture
References
Appendix A - Equipment
Appendix B - Appearance of Uninoculated Monolayers and CPE
             Produced by Selected Viruses
The Quality System Approach
Related CLSI/NCCLS Publications

Provides guidance for viral culture and identification procedures performed in the clinical virology laboratory.