CLSI M42 P : 1ED 2005

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Definitions
4 Indications for Performing Susceptibility Testing
5 Selection of Antimicrobial Agents for Routine Testing and
  Reporting
  5.1 Routine Reports
  5.2 Antimicrobial Classes
  5.3 Suggested Guidelines for Use and Selective Testing and
      Reporting
6 Disk Diffusion Assay Protocol
  6.1 Media Preparation
  6.2 Inoculum
  6.3 Diffusion Disks
  6.4 Incubation
  6.5 Reading Plates
  6.6 Rejection Criteria
7 Fastidious and Problem Organisms
  7.1 Obligate Halophilic Strains (Group 2)
  7.2 Gliding Bacteria (Group 3)
  7.3 Streptococci (Group 4)
  7.4 Other Fastidious Organisms (Group 5)
8 Quality Control Procedures
  8.1 Purpose
  8.2 Reference Strains for Quality Control
  8.3 Accepted Quality Controls Ranges
  8.4 Expansion of Control Strain Set
  8.5 Storing and Testing Quality Control Strains
  8.6 Control of Media and Disks
  8.7 Frequency of Quality Control Testing
  8.8 Corrective Action
9 Quality Assurance Measures
References
Appendix A. Antimicrobial Agents Used in Global Aquaculture
            and Status of Quality Control for Disk Diffusion
            Susceptibility Testing
Appendix B. Fuzzy Zones and Halo Formation
Appendix C. Disk Diffusion Daily Quality Control Testing
            Protocol
Appendix D. Disk Diffusion Weekly Quality Control Testing
            Protocol
Tables
The Quality System Approach
Related CLSI/NCCLS Publications

Describes the standard agar disk diffusion method and quality control criteria for testing Group 1 aquatic bacteria.