CLSI M42 R : 1ED 2003

Abstract
Committee Membership
Foreword and Scope
1 Introduction
2 Definitions
3 Disk Diffusion Assay Protocol
   3.1 Media Preparation
   3.2 Inoculum
   3.3 Diffusion Disks
   3.4 Incubation
   3.5 Reading Plates
   3.6 Rejection Criteria
4 Quality Control Procedures
   4.1 Purpose
   4.2 Reference Strains for Quality Control
   4.3 Accepted Quality Control Limits
   4.4 Expansion of Control Strain Set
   4.5 Storing Quality Control Strains
   4.6 Control of Media and Disks
   4.7 Frequency of Quality Control Testing
   4.8 Corrective Action
5 Quality Assurance Measures
References
Appendix A Disk Contents
Appendix B Recommended Grouping for Disk Diffusion
           Susceptibility Testing
Appendix C Fuzzy Zones and Halo Formation
Appendix D Disk Diffusion Daily Quality Control Testing
           Protocol
Appendix E Disk Diffusion Weekly Quality Control testing
           Protocol
Appendix F Glossary of Related Terms
Tables
The Quality System Approach
Related NCCLS Publications

Gives the most up-to-date techniques for disk diffusion susceptibility testing of aquatic species isolates, and criteria for quality control testing.