CLSI M44 P : 1ED 2003
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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METHOD FOR ANTIFUNGAL DISK DIFFUSION SUSCEPTIBILITY TESTING OF YEASTS
20-05-2004
12-01-2013
Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Selection of Antimicrobial Agent Disks for Routine
Testing and Reporting
5.1 Use of Nonproprietary or Generic Names
5.2 Number of Agents Tested
5.3 Suggested Guidelines for Selective Reporting
6 Equipment/Materials
7 Reagents for the Disk Diffusion Test
7.1 Mueller-Hinton Agar + 2% Glucose and 0.5
Miligram/mL Methylene Blue Dye (GMB) Medium
7.2 Turbidity Standard for Inoculum Preparation
8 Procedure for Performing the Disk Diffusion Test
8.1 Inoculum Preparation: Direct Colony Suspension
Method
8.2 Inoculation of Tests Plates
8.3 Application of Disks to inoculated Agar Plates
8.4 Reading Plates and Interpreting Results
9 Interpretation of Disk Diffusion Test Results
9.1 Zone Diameter Interpretive Standards
9.2 Interpretive Categories
9.3 Equivalent MIC Breakpoints
10 Quality Control Procedures
10.1 Purpose
10.2 Reference Strains for Quality Control
10.3 Storing Quality Control Strains
10.4 Zone Diameter Quality Control Limits
10.5 Frequency of Quality Control Testing
10.6 Corrective Action
10.7 Reporting Patient Results When Out-of-Control
Tests Occur
11 Limitations of Disk Diffusion Methods
11.1 Application to Various Organism Groups
11.2 Verification of Patient Results
References
Table 1 Zone Diameter Interpretive Standards and
Equivalent Minimal Inhibitory Concentrations (MIC)
Breakpoints for Candida species
Table 2 Recommended Quality Control Zone Diameter (mm)
Ranges
Appendix A Preparation of Mueller-Hinton Agar + 2%
Glucose and 0.5 Microgram/mL Methylene
Blue Dye
Appendix B McFarland 0.5 Barium Sulfate Turbidity
Standard
Appendix C Quality Control protocol Flow Charts
The Quality System Approach
Related NCCLS Publications
Gives newly established methodology for disk diffusion testing of Candida spp., criteria for quality control testing, and interpretive criteria.
DocumentType |
Proposed Guideline
|
PublisherName |
Clinical Laboratory Standards Institute
|
Status |
Superseded
|
SupersededBy |
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