CLSI M49 P : 1ED 2005

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Definitions
4 Antimicrobial Agents
   4.1 Source
   4.2 Weighing Antimicrobial Powders
   4.3 Preparing Stock Solutions
   4.4 Number of Concentrations Tested
5 Selection of Antimicrobial Agents for Routine Testing
   and Reporting
   5.1 Routine Reports
   5.2 Antimicrobial Classes
   5.3 Suggested Guidelines for Use and Selective Testing
        and Reporting
6 Indications for Performing Susceptibility Testing
7 Inoculum Preparation Dilution Tests
   7.1 Turbidity Standard for Inoculum Preparation
   7.2 Direct Colony Suspension Method
8 Broth Dilution Procedures (Macrodilution and Microdilution)
   8.1 Cation-Adjusted Mueller-Hinton Broth (CAMBH) Medium
   8.2 Preparing and Storing Diluted Antimicrobial Agents
   8.3 Broth Dilution Testing
9 Fastidious and Problem Organisms
   9.1 Obligate Halphilic Strains (Group 2)
   9.2 Gliding Bacteria (Group 3)
   9.3 Streptococci (Group 4)
   9.4 Other Fastidious Organisms (Group 5)
10 Reporting of MIC Results
11 Quality Control Procedures
   11.1 Purpose
   11.2 Quality Control Responsibilities
   11.3 Reference Strains for Quality Control
   11.4 Storing and Testing Quality Control Strains
   11.5 Control of Media and Materials
   11.6 Broth Dilution Quality Control Ranges
   11.7 Frequency of Quality Control Testing
   11.8 Corrective Action
   11.9 Other Control Procedures
12 Limitations of Broth Dilution Test Methods
   12.1 Application to Various Organism Groups
References
Appendix A. Antimicrobial Agents Used in Global Aquaculture
            and Status of Quality Control for Broth Dilution
            Susceptibility Testing
Appendix B. Aerobic Dilution Daily Quality Control Testing
            Protocol
Appendix C. Aerobic Dilution Weekly Quality Control Testing
            Protocol
The Quality System Approach
Related CLSI/NCCLS Publications

Describes the most up-to-date techniques for the determination of minimal inhibitory concentrations (MICs) of aquatic bacteria by broth micro-and macrodilution, and criteria for quality control testing.