CLSI M51 A : 1ED 2010

Abstract
Committee Membership
Foreword
1 Scope
2 Standard Precautions
3 Terminology
4 Selection of Antimicrobial Agent Disks for Routine
   Testing and Reporting
5 Equipment/Materials
6 Test Procedures
7 Procedure for Performing the Disk Diffusion Test
8 Interpretation of Disks Diffusion Test Results
9 Quality Control Procedures
10 Limitations of Disk diffusion Method
References
Appendix A - Performance of Nonsupplemented Muller-Hinton
             Agar
Appendix B - Preparation of Nonsupplemented Muller-Hinton
             agar
Appendix C - McFarland 0.5 Barium Sulfate Turbidity
             Standard
Appendix D - Quality Control Protocol Flow Charts
Summary of Delegate Comments and Subcommittee Responses
The Quality Management System Approach
Related CLSI Reference Materials

Gives an established method for disk diffusion testing of moulds, zone interpretive criteria, and recommended control ranges for amphotericin B, caspo fungin, itraconazole, posaconazole, and voriconazole.

With a need to make antifungal susceptibility testing more readily available to the clinical laboratory, this CLSI document provides an established method for disk diffusion testing of moulds, zone interpretive criteria, and recommended control ranges for amphotericin B, caspofungin, itraconazole, posaconazole, and voriconazole. The method described in this document is intended for testing moulds that cause invasive disease (Alternaria spp., Aspergillus spp., Bipolaris spp., Fusarium spp., Paecilomyces spp., Rhizopus oryzae [R. arrhizus] and other mucoraceous [zygomycetes] mould species, the Pseudallescheria boydii species complex, and Scedosporium prolificans).4 This method does not currently encompass the yeast or mould
form of endemic dimorphic fungi or the dermatophytes. The method described herein must be followed exactly to obtain reproducible results. When new problems are recognized or improvements in these criteria are developed, changes will be incorporated into future editions of this guideline and also distributed in periodic informational supplements. This guideline is intended for use by, but not limited to, health care, academic, government, industry, or independent research organizations that perform antifungal susceptibility testing of filamentous fungi.