CLSI M54 A : 2012

Abstract
Committee Membership
Foreword
1 Scope
2 Standard Precautions
3 Terminology
4 Biosafety
5 Levels of Laboratory and Referral Services
6 Quality Control and Maintenance of Stock Cultures
7 Specimen Collection and Transport
8 Specimen Processing
9 Direct Examination of Clinical Specimens
10 Specimen Plating and Incubation
11 Culture Assessment
References
Appendix A - Specimen Collection, Transport,
             and Processing Chart
Appendix B - Principles of Fluorescent Microscopy
Appendix C - Histopathological Stains
Appendix D - Control of Mite Infestations in Fungal Cultures
Appendix E - Additional Isolation Media for Culturing
             Sputum and Other Respiratory Tract Samples
             From Cystic Fibrosis Patients
The Quality Management System Approach
Related CLSI Reference Materials

Specifies protocols, quality control parameters, and interpretive criteria for performing fungal cultures and for the detection and identification of fungi in direct examinations.

Mycologists have recognized the need for a consensus guideline that serves as a concise reference for the performance of fungal culturing and the preparation and reading of direct microscopic examinations. M54 provides principles and procedures for biosafety in the mycology laboratory, QC of fungal media, maintenance of stock strains, specimen collection and transport, specimen processing, direct microscopic examinations, fungal culturing methods, and assessment of cultures.This guideline is intended for laboratory scientists who process specimens for fungal culture and/or perform fungal direct microscopic examinations. Although they can be encountered growing on mycology media, Nocardia spp. and other aerobic actinomycetes can also be found growing on media in other areas of the laboratory (eg, bacteriology, mycobacteriology). Therefore, culture and direct examinations for this group of organisms will not be discussed in this document. Furthermore, the identification of fungi is outside of the scope of this document.