CLSI MM16 P : 1ED 2005
Superseded
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USE OF EXTERNAL RNA CONTROLS IN GENE EXPRESSION ASSAYS
29-08-2006
12-01-2013
Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Standard Precautions
4 Terminology
4.1 Definitions
4.2 Acronyms/Abbreviations
5 Overview
5.1 Gene Expression Profiling Technology
5.2 Gene Expression Profiling in Clinical Applications
5.3 A Note on Technical Performance and Clinical
Performance
6 External RNA Controls and Their Use in Expression Assays
6.1 Internal and External RNA Controls in Gene Expression
Assays
6.2 Characteristics of External RNA Controls
6.3 Using Control Materials to Assess Technical and
Clinical Performance
6.4 Limitations of External Controls
6.5 Metrics for Assessing Technical Performance of the
Expression Platform
7 Recommended Practices/Protocols
7.1 Overview
7.2 Protocols for Preparation and Assessment of External
RNA Controls
7.3 External RNA Control Protocols for Microarrays
7.4 External RNA Control Protocols for QRT-PCR
References
The Quality System Approach
Related CLSI/NCCLS Publications
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