CLSI MM18 A : 1ED 2008

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Standard Precautions
4 Terminology
  4.1 Definitions
  4.2 Abbreviations and Acronyms
5 Approach to DNA Target Sequencing
  5.1 Primer Design
  5.2 Controls
  5.3 Protocol and Instrument Requirements
  5.4 Overview of Sequence Data
  5.5 Selection of Reference Databases
  5.6 Sequence Comparison for Identification
  5.7 Generalized Approach to Sequence-Based Identification
       for Bacteria (excluding Mycobacteria and Actinomycetes)
6 Interpretive Criteria of Identity Scores
  6.1 Staphylococci and Related Gram-Positive Cocci
  6.2 Streptococcus spp., Enterococcus spp., and
       Streptococcal-Like Organisms
  6.3 Glucose Nonfermenting Gram-Negative Bacilli
  6.4 Campylobacterales
  6.5 Gram-Positive Anaerobes
  6.6 Gram-Negative Anaerobes
  6.7 Coryneform Gram-Positive Bacilli
  6.8 Aerobic Actinomycetes
  6.9 Mycobacterium sp.
  6.10 Bacterial Agents of Bioterrorism
  6.11 Fungi
7 Suggestions for Result Reporting
References
Summary of Delegate Comments and Committee Responses
The Quality Management System Approach
Related CLSI Reference Materials

Specifies recommendations for clinical laboratories that employ amplification and Sangerbased (dideoxy-termination) sequencing of broad-range DNA targets for the identification of bacteria, mycobacteria, and fungi from cultured clinical isolates.