DD ISO/TS 23893-2:2007

Foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 Test environment
5 Reagents
6 Apparatus
7 Sampling and preparation of samples
   7.1 Sampling of fish
   7.2 Killing of fish and dissection
   7.3 Storage
8 Procedure
   8.1 Preparation of the fractions
   8.2 Determination of protein
   8.3 Determination of the EROD activity
   8.4 Checking the sensitivity of the biological reagent and
       compliance with the operating method
9 Expression of results
   9.1 Calculation of the EROD activity
   9.2 Statistical processing of the data
10 Test report
Annex A (informative) - Examples of results
Bibliography

Defines a method for measuring the ethoxyresorufin-O-deethylase (EROD) enzyme activity on a post-mitochondrial fraction of fish liver homogenate (subcellular fraction in which the EROD activity is located) employing a cell or microplate fluorimetric method.

This part of ISO23893 specifies a method for measuring the ethoxyresorufin-O-deethylase (EROD) enzyme activity on a post-mitochondrial fraction of fish liver homogenate (subcellular fraction in which the EROD activity is located) employing a cell or microplate fluorimetric method.

It applies to fish that are sampled in their natural environment (fresh water or salt water) or exposed to substances or effluents in a laboratory.

This method is applicable for EROD values greater than or equal to 1pmol/(min·mg) of proteins. A higher sensitivity may be achieved by using a cell (test tube) procedure.