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DIN EN 16418 E : 2014

Current

Current

The latest, up-to-date edition.

PAPER AND BOARD - DETERMINATION OF THE CYTOTOXICITY OF AQUEOUS EXTRACTS USING A METABOLICALLY COMPETENT HEPATOMA CELL LINE (HEPG2)

Published date

30-09-2014

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National foreword<br>National Annex NA (informative) - Bibliography<br>Foreword<br>1 Scope<br>2 Normative references<br>3 Terms and definitions<br>4 Principle<br>5 Reagents<br>6 Cell line<br>7 Food simulants used for testing<br>8 Cleaning laboratory glassware<br>9 Equipment<br>10 Preparation of specimens<br>11 Cytotoxicity assessment<br>12 Expression of the results<br>13 Interpretation of the results<br>14 Precision<br>15 Test report<br>Annex A (informative) - 96-well plates configuration<br>Annex B (informative) - RNA Synthesis rate inhibition<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;cytotoxicity test work flow<br>Annex C (informative) - Validation of the two methods<br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(option A and B)<br>Bibliography

Defines a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG2 cell line.

DocumentType
Standard
PublisherName
German Institute for Standardisation (Deutsches Institut für Normung)
Status
Current

Standards Relationship
EN 16418:2014 Identical

EN 645:1993 Paper and board intended to come into contact with foodstuffs - Preparation of a cold water extract
EN 15845:2010 Paper and board - Determination of the cytotoxicity of aqueous extracts
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
EN 647:1993 Paper and board intended to come into contact with foodstuffs - Preparation of a hot water extract
DIN ISO 3696:1991-06 WATER FOR ANALYTICAL LABORATORY USE - SPECIFICATION AND TEST METHODS

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