DIN EN ISO 22174:2005-05
Current
The latest, up-to-date edition.
MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
Hardcopy , PDF
English
01-01-2005
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Preliminary microbial enrichment
4.3 Nucleic acid preparation
4.4 PCR amplification
4.5 Detection and confirmation of PCR products
5 Test material
6 General laboratory requirements
6.1 General
6.2 Personnel
6.3 Laboratory setup
6.4 Waste management
7 Reagents
8 Apparatus and equipment
8.1 General
8.2 Special considerations
9 Procedure
9.1 Sample preparation
9.2 Amplification
9.3 Control reaction
9.4 Confirmation of PCR results
10 Evaluation
11 Test report
Bibliography
Describes the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). Applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR).
| DevelopmentNote |
Supersedes DIN 10134 (05/2005)
|
| DocumentType |
Standard
|
| Pages |
16
|
| PublisherName |
German Institute for Standardisation (Deutsches Institut für Normung)
|
| Status |
Current
|
| Supersedes |
| Standards | Relationship |
| BS EN ISO 22174:2005 | Identical |
| NBN EN ISO 22174 : 2005 | Identical |
| EN ISO 22174:2005 | Identical |
| NS EN ISO 22174 : 1ED 2005 | Identical |
| UNE-EN ISO 22174:2005 | Identical |
| NEN EN ISO 22174 : 2005 | Identical |
| NF EN ISO 22174 : 2005 | Identical |
| ISO 22174:2005 | Identical |
| UNI EN ISO 22174 : 2005 | Identical |
| I.S. EN ISO 22174:2005 | Identical |
| DIN EN ISO 15216-1:2015-09 (Draft) | MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO 15216-1:2017) |
| DIN EN ISO 22118:2011-11 | Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens - Performance characteristics (ISO 22118:2011) |
| DIN EN ISO 22119:2011-11 | Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011) |
| ISO/IEC 17025:2005 | General requirements for the competence of testing and calibration laboratories |
| ISO 5725-1:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions |
| ISO 20837:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for sample preparation for qualitative detection |
| ISO 20838:2006 | Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods |
| ISO 3534-1:2006 | Statistics — Vocabulary and symbols — Part 1: General statistical terms and terms used in probability |
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