EN 1104:2005

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
   5.1 Punch iron
   5.2 Pressing device
   5.3 Zone reading device
   5.4 Ordinary microbiological laboratory apparatus
6 Reagents
   6.1 Water
   6.2 Non ionic wetting agent,
   6.3 Nutrient medium for Bacillus subtilis
   6.4 Sabouraud modified mould nutrient medium for Aspergillus niger
   6.5 Nutrient medium for inhibition test with Bacillus subtilis
   6.6 Salt peptone solution
   6.7 Test micro-organisms
        6.7.1 General
        6.7.2 Preparation of inoculating spore suspension of
               Bacillus subtilis
        6.7.3 Preparation of inoculating spore suspension of
               Aspergillus niger
        6.7.4 Concentrations of spores for inhibition test
   6.8 Positive controls
        6.8.1 Penicillin, G 0,03 units
        6.8.2 Isothiazolinon-solution
7 Sampling and preparation of test pieces
8 Procedure
   8.1 Sterilisation
   8.2 Preparation of plates
        8.2.1 General
        8.2.2 Bacillus subtilis
        8.2.3 Aspergillus niger
   8.3 Incubation
9 Evaluation
10 Test report

This European Standard specifies a method for the determination of transfer of antimicrobial constituents from paper and board materials and articles intended for food contact.