EN 14122:2014

Foreword
1 Scope
2 Normative references
3 Principles
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Examples of HPLC chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Alternative HPLC systems
Annex D (informative) - Vitamin B1 compound: 2-
        (1-hydroxyethyl)thiamin (HET) performing
        post-column derivatization
Bibliography

This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig's liver ranging from 0,295 mg/100 g to 0,807 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/100 g to 486 mg/100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives.For further information on the validation, see Clause 8 and Annex B.