EN 14164:2014
Current
The latest, up-to-date edition.
Foodstuffs - Determination of vitamin B6 by high performance chromatography
04-06-2014
Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Test report
Annex A (informative) - Example of a chromatogram
Annex B (informative) - Precision data
Annex C (informative) - Sample treatment option without
acid hydrolysis
Annex D (informative) - Examples for molar absorption
coefficients
Bibliography
This European Standard specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The β-glycosylated forms are not taken into account. These can be determined with the method given in EN 14663 [1] by which the different vitamins of vitamin B6 (pyridoxal, pyridoxamine and pyridoxine) are separated and individually quantified. A third European Standard, EN 14166 [2], determines the total vitamin B6 by microbiological assay.
| Committee |
CEN/TC 275
|
| DevelopmentNote |
Supersedes ENV 14164. (07/2008) Supersedes PREN 14164. (06/2014)
|
| DocumentType |
Standard
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Current
|
| Supersedes |
| Standards | Relationship |
| NF EN 14164 : 2014 | Identical |
| NEN EN 14164 : 2014 | Identical |
| NBN EN 14164 : 2014 | Identical |
| NS EN 14164 : 2014 | Identical |
| I.S. EN 14164:2014 | Identical |
| UNE-EN 14164:2014 | Identical |
| UNI EN 14164 : 2014 | Identical |
| DIN EN 14164:2014-08 | Identical |
| BS EN 14164:2014 | Identical |
| SN EN 14164:2014 | Identical |
| PN EN 14164 : 2014 | Identical |
| ISO/IEC 17025:2005 | General requirements for the competence of testing and calibration laboratories |
| EN 14663:2005 | Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC |
| ISO 5725:1986 | Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
| EN 14166:2009 | Foodstuffs - Determination of vitamin B6 by microbiological assay |
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