EN 14524:2004
Withdrawn
A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.
Foodstuffs - Determination of okadaic acid in mussels - HPLC method with solid phase extraction clean-up, derivatization and fluorimetric detection
21-08-2009
11-08-2004
Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents and materials
5 Apparatus
6 Sampling
7 Procedure
8 Precision
9 Test report
Annex A (informative) Typical chromatogram
Annex B (informative) Precision data
Bibliography
This European Standard specifies a method for the quantitative determination of the content of okadaic acid in mussels and mussel products. The content of okadaic acid is determined as free extractable acid of mussel hepatopancreas. Okadaic acid, a fat-soluble toxin from dinophysis algae, is a main component of dinophysis toxins.The method has been validated in an interlaboratory study according to ISO general principles on assessing accuracy of measurement methods and results. The limit of determination of this method (signal/noise = 10) is 100 µg/kg for okadaic acid in mussel hepatopancreas. The method has been validated for okadaic acid in cooked mussels at levels of 441 µg/kg to 1 467 µg/kg.Laboratory experiences have shown that this method can also be used to determine other dinophysis toxins, e.g. dinophysis toxins 1, 2 and 3 (DTX-1, DTX-2 and DTX-3), see [1], [2], [3], [4], [5] and [6]
| Committee |
CEN/TC 275
|
| DocumentType |
Standard
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Withdrawn
|
| Standards | Relationship |
| NBN EN 14524 : 2004 | Identical |
| NEN EN 14524 : 2004 | Identical |
| NS EN 14524 : 2004 | Identical |
| I.S. EN 14524:2004 | Identical |
| PN EN 14524 : 2006 | Identical |
| UNI EN 14524 : 2005 | Identical |
| UNE-EN 14524:2005 | Identical |
| BS EN 14524:2004 | Identical |
| NF EN 14524 : 2004 | Identical |
| DIN EN 14524 E : 2004 | Identical |
| DIN EN 14524:2004-10 | Identical |
| ISO 5725-1:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions |
| ISO 5725-6:1994 | Accuracy (trueness and precision) of measurement methods and results — Part 6: Use in practice of accuracy values |
| ISO 5725-4:1994 | Accuracy (trueness and precision) of measurement methods and results Part 4: Basic methods for the determination of the trueness of a standard measurement method |
| ISO 5725-2:1994 | Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
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