EN 14663:2005
Current
The latest, up-to-date edition.
Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC
07-12-2005
Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) Precision data
Annex B (informative) Examples for suitable HPLC-conditions
for the determination of vitamin B[6]
compounds
Annex C (informative) Examples for molar extinction coefficients
Annex D (informative) Figures
Bibliography
This document specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC).Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives as well as the b-glycosylated forms that have been added to foodstuffs, calculated as pyridoxine.This method has been successfully validated with semolina with milk (infant food), potato puree, vegetables with ham (convenient products) and a multi vitamin drink at levels from 0,034 mg/100 g to 1,21 mg/100 g.
| Committee |
CEN/TC 275
|
| DocumentType |
Standard
|
| PublisherName |
Comite Europeen de Normalisation
|
| Status |
Current
|
| Standards | Relationship |
| NF EN 14663 : 2006 | Identical |
| NS EN 14663 : 1ED 2006 | Identical |
| SN EN 14663 : 2006 | Identical |
| UNE-EN 14663:2007 | Identical |
| I.S. EN 14663:2006 | Identical |
| UNI EN 14663 : 2006 | Identical |
| NBN EN 14663 : 2006 | Identical |
| NEN EN 14663 : 2005 | Identical |
| BS EN 14663:2005 | Identical |
| PN EN 14663 : SEP 2006 | Identical |
| DIN EN 14663:2006-03 | Identical |
| UNE-EN 14663:2005 | Identical |
| DIN EN 14164:2014-08 | FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HIGH PERFORMANCE CHROMATOGRAPHY |
| 07/30163680 DC : 0 | BS EN 14164 - FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HPLC |
| PREN 14164 : DRAFT 2012 | FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HPLC |
| 12/30258767 DC : 0 | BS EN 14164 - FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HPLC |
| I.S. EN 14164:2014 | FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HIGH PERFORMANCE CHROMATOGRAPHY |
| BS EN 14164:2014 | Foodstuffs. Determination of vitamin B6 by high performance chromatography |
| UNE-EN 14164:2014 | Foodstuffs - Determination of vitamin B6 by high performance chromatography |
| UNI EN 14164 : 2014 | FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HIGH PERFORMANCE CHROMATOGRAPHY |
| EN 14164:2014 | Foodstuffs - Determination of vitamin B6 by high performance chromatography |
| ISO 5725:1986 | Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
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