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EN 16877:2016

Current

Current

The latest, up-to-date edition.

Animal feeding stuffs: Methods of sampling and analysis - Determination of T-2 and HT-2 toxins, Deoxynivalenol and Zearalenone, in feed materials and compound feed by LC-MS

Published date

30-11-2016

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European foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedures
7 Measurements
8 Determination of mass fraction
9 Precision
10 Test report
Annex A (informative) - Precision data
Annex B (informative) - Examples
Annex C (informative) - Examples of chromatograms
        according to the settings of the examples in Annex B
Bibliography

This method of analysis is applicable to the determination of HT-2 toxin (HT2) in the tested range of 22 µg/kg to 178 µg/kg, T-2 toxin (T2) in the tested range of 7 µg/kg to 50 µg/kg, Deoxynivalenol (DON) in the tested range of 88 µg/kg to 559 µg/kg, and Zearalenone (ZON) in the tested range of 14 µg/kg to 430 µg/kg in cereals and cereal-based compound animal feed. The actual working ranges may extend beyond the tested ranges. It is the responsibility of the laboratory to prove that the limit of quantitation (LOQ) for HT-2 and T-2 toxin is ≤ 10 µg/kg, for DON ≤ 100 µg/kg, and for ZON ≤ 20µg/kg.

Committee
CEN/TC 327
DevelopmentNote
Supersedes PREN 16877. (12/2016)
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

Standards Relationship
DIN EN 16877:2017-03 Identical
NF EN 16877 : 2016 Identical
NS EN 16877 : 2016 Identical
PN EN 16877 : 2017 Identical
SN EN 16877:2017 Identical
I.S. EN 16877:2016 Identical
UNI EN 16877 : 2017 Identical
UNE-EN 16877:2017 Identical
DIN EN 16877:2015-07 (Draft) Identical
BS EN 16877:2016 Identical
NEN EN 16877 : 2016 Identical

EN ISO 6498:2012 Animal feeding stuffs - Guidelines for sample preparation (ISO 6498:2012)
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 6498:2012 Animal feeding stuffs — Guidelines for sample preparation
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
ISO 5725-5:1998 Accuracy (trueness and precision) of measurement methods and results — Part 5: Alternative methods for the determination of the precision of a standard measurement method

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