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EN 17270:2019

Current

Current

The latest, up-to-date edition.

Animal feeding stuffs: Methods of sampling and analysis - Determination of theobromine in feed materials and compound feed, including cocoa derived ingredients, by liquid chromatography

Published date

23-10-2019

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This document method is applicable for the determination of theobromine in compound feed by liquid chromatography with UV detection in the tested range of 27 to 307 mg/kg. This method has been validated using complementary compound feed for adult dogs and complementary compound feedstuff for horses. The actual working range may extend beyond the tested range. Alternative chromatography conditions using liquid chromatography tandem mass spectrometry (LC-MS/MS) are also provided for the validated range of 49 to 307 mg/kg. This method has also been shown to be fit for purpose for the determination of theobromine in baking chocolate by both HPLC-UV and LC-MS/MS.

Committee
CEN/TC 327
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

Standards Relationship
DIN EN 17270:2019-12 Identical
NF EN 17270:2019 Identical
I.S. EN 17270:2019 Identical
PN-EN 17270:2020-03 Identical
BS EN 17270:2019 Identical
ÖNORM EN 17270: 2020 01 01 Identical
NS-EN 17270:2019 Identical
UNE-EN 17270:2020 Identical

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