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  • EN ISO 11701:2009

    Current The latest, up-to-date edition.

    Vegetable fats and oils - Determination of phospholipids content in lecithins by HPLC using a light-scattering detector (ISO 11701:2009)

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    Published date:  15-12-2009

    Publisher:  Comite Europeen de Normalisation

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    Table of Contents - (Show below) - (Hide below)

    Foreword
    1 Scope
    2 Normative references
    3 Terms and definitions
    4 Principle
    5 Reagents
    6 Apparatus
    7 Sampling
    8 Preparation of test sample
    9 Procedure
    10 Calculation and expression of results
    11 Precision of the method
    12 Test report
    Annex A (informative) - HPLC chromatogram
    Annex B (informative) - Results of an interlaboratory
                            test
    Bibliography

    Abstract - (Show below) - (Hide below)

    ISO 11701:2009 specifies a method for the quantitative determination of phospholipids content by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector.The method is applicable to crude, oil-containing lecithins, and to oil-free lecithins and lecithin fractions from vegetable fats and oils.The method is not applicable to animal and ruminant lecithins and enzymatically hydrolysed lecithins as the peak separation of lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient.

    General Product Information - (Show below) - (Hide below)

    Committee CEN/TC 307
    Document Type Standard
    Publisher Comite Europeen de Normalisation
    Status Current

    Standards Referencing This Book - (Show below) - (Hide below)

    ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
    ISO 5555:2001 Animal and vegetable fats and oils — Sampling
    ISO 1042:1998 Laboratory glassware One-mark volumetric flasks
    ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
    ISO 661:2003 Animal and vegetable fats and oils Preparation of test sample
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