EN ISO 11731:2017

European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus and glassware
6 Culture media and reagents
7 Sampling
8 Procedure
9 Expression of results
10 Test report
11 Quality assurance
Annex A (informative) - Legionella species
Annex B (normative) - Culture media
Annex C (normative) - Diluents
Annex D (normative) - Acid solution
Annex E (informative) - Scraping or rubbing the
        bacteria from membrane filters
Annex F (informative) - Centrifugation technique
Annex G (informative) - Indirect immunofluorescent
        antibody assay for the identification of Legionella
        species
Annex H (informative) - Performance data
Annex I (informative) - Pre-treatment of water related
        matrices
Annex J (normative) - Decision matrix
Bibliography

ISO 11731:2017 specifies culture methods for the isolation of Legionella and estimation of their numbers in water samples.These methods are applicable to all kinds of water samples including potable, industrial, waste and natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc.Not all Legionella species are culturable; therefore, the methods described in this document do not recover all species of Legionella.