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EN ISO 14939:2001

Current

Current

The latest, up-to-date edition.

ANIMAL FEEDING STUFFS - DETERMINATION OF CARBADOX CONTENT - METHOD USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Published date

15-08-2001

Foreword
1 Scope
2 Normative reference
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparation of test sample
8 Procedure
   8.1 General
   8.2 Preparation of spiked sample
   8.3 Extraction
   8.4 Column chromatography
   8.5 HPLC analysis
9 Confirmation
   9.1 General
   9.2 Co-chromatography
   9.3 Diode array detector
   9.4 Post-column derivatization
10 Calculation of results
   10.1 General
   10.2 Feeding stuffs containing 0,1 mg/kg to 10 mg/kg
        of carbadox
   10.3 Feeding stuffs containing 0,1 mg/kg to 100 mg/kg
   10.4 Premixtures containing up to 10 % of carbadox
11 Precision
   11.1 Interlaboratory test
   11.2 Repeatability
   11.3 Reproducibility
12 Test report
Annex A (informative) Flow chart
Annex B (informative) Results of interlaboratory test
Bibliography

Describes a high-performance liquid chromatographic (HPLC) method for the determination of the carbadox content in premixtures and animal feeding stuffs.

Committee
TC 327
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 6497:2002 Animal feeding stuffs Sampling
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 6498:2012 Animal feeding stuffs — Guidelines for sample preparation

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