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CEN
EN ISO 15788-2 : 2004

EN ISO 15788-2 : 2004

Current

Current

The latest, up-to-date edition.

ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF STIGMASTADIENES IN VEGETABLE OILS - PART 2: METHOD USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Published date

17-11-2004

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
   8.1 General
   8.2 External standard method
   8.3 Internal standard method
9 Procedure
   9.1 High-pressure liquid chromatography (HPLC)
   9.2 Identification of steradienes
10 Expression of results
   10.1 External standard
   10.2 Internal standard
11 Precision
   11.1 Interlaboratory test
   11.2 Repeatability
   11.3 Reproducibility
12 Test report
Annex A (informative) Results of interlaboratory test
Annex B (informative) Examples of chromatograms
Bibliography

Defines a method for the determination of steradienes, especially stigmastadienes. Steradienes are formed by dehydration of sterols during bleaching and also partially during steam washing and deodorization.

Committee
TC 307
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 5555:2001 Animal and vegetable fats and oils — Sampling
ISO 12228:1999 Animal and vegetable fats and oils Determination of individual and total sterols contents Gas chromatographic method
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods

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