• There are no items in your cart

EN ISO 20418-1:2018

Current

Current

The latest, up-to-date edition.

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 1: Peptide detection using LC-ESI-MS with protein reduction (ISO 20418-1:2018)

Published date

16-05-2018

Sorry this product is not available in your region.

European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Reagents
6 Apparatus
7 Test method
Annex A (informative) - Parameters
Annex B (informative) - Markers
Annex C (informative) - Examples of interlaboratory results
Bibliography

ISO 20418-1:2018 specifies a qualitative and quantitative testing method to determine the content of wool, cashmere, yak fibres and their blends in textiles by microscope preliminary screening, protein extraction, enzymatic digestion and specific peptides detection using a liquid chromatography-mass spectrometer equipped with electrospray ionization source (LCI-ESI-MC).This method can be applied to relevant textile products at each process stage (i.e. from raw material to garment) with a homogeneous distribution of the components. It can be applied to different types of textile materials (e.g. staples, tops, yarns and fabrics) that contain wool, cashmere or yak fibres and their blends. The method is based on a preliminary identification of all fibres in the blend on the basis of their morphology, by light microscopy. The proteins are then extracted by a thiourea/urea/dithiothreitol (DTT) solution. An enzymatic digestion by trypsin of the protein extracted from the fibres is carried out. Analysis of the specific markers is performed by LC-MS and the percent composition is calculated.This method is applicable to samples containing other kinds of fibres than wool, cashmere and yak, by combining its results with the results obtained using the ISO 1833 series and/or the ISO 17751 series.ISO 20418-1:2018 does not apply if fibres of the same animal species are present (e.g. blends of cashmere and mohair); in this case, the quantitative analysis can be performed using microscopic analysis (e.g. ISO 17751 series).

Committee
CEN/TC 248
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

ISO 13528:2015 Statistical methods for use in proficiency testing by interlaboratory comparison
ISO/TR 11827:2012 Textiles — Composition testing — Identification of fibres
ISO 2647:1973 Wool Determination of percentage of medullated fibres by the projection microscope
ISO 137:2015 Wool Determination of fibre diameter Projection microscope method
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method

Access your standards online with a subscription

Features

  • Simple online access to standards, technical information and regulations.

  • Critical updates of standards and customisable alerts and notifications.

  • Multi-user online standards collection: secure, flexible and cost effective.