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EN ISO 20837:2006

Withdrawn

Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection (ISO 20837:2006)

Withdrawn date

31-10-2006

Published date

15-04-2006

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Foreword
Introduction
1 Scope
2 Normative references
3 Principle
4 General laboratory requirements
5 Reagents, apparatus and equipment
6 Procedure
Annex A (informative) Standards concerning the enrichment of
                      microorganisms (bacteria)
Annex B (informative) Method for DNA extraction from
                      Gram-negative bacteria
Bibliography

ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B. ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.

Committee
CEN/TC 463
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Withdrawn

ISO 22174:2005 Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions
ISO 6579:2002 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Salmonella spp.
ISO 21567:2004 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Shigella spp.
ISO 11290-1:2017 Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 1: Detection method
ISO/TS 20836:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance testing for thermal cyclers
ISO 7937:2004 Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of Clostridium perfringens — Colony-count technique
ISO 10272-1:2017 Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method
ISO 6888-3:2003 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part 3: Detection and MPN technique for low numbers
ISO 7932:2004 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of presumptive Bacillus cereus Colony-count technique at 30 degrees C
ISO 10273:2017 Microbiology of the food chain — Horizontal method for the detection of pathogenic Yersinia enterocolitica
ISO 20838:2006 Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods
ISO 16654:2001 Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157
DIN 10135:2013-05 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - METHOD FOR THE DETECTION OF SALMONELLA

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