EN ISO 22174:2005

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 General
   4.2 Preliminary microbial enrichment
   4.3 Nucleic acid preparation
   4.4 PCR amplification
   4.5 Detection and confirmation of PCR products
5 Test material
6 General laboratory requirements
   6.1 General
   6.2 Personnel
   6.3 Laboratory setup
   6.4 Waste management
7 Reagents
8 Apparatus and equipment
   8.1 General
   8.2 Special considerations
9 Procedure
   9.1 Sample preparation
   9.2 Amplification
   9.3 Control reaction
   9.4 Confirmation of PCR results
10 Evaluation
11 Test report
Bibliography

ISO 22174:2004 gives the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). It is applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR)The minimum requirements laid down in ISO 22174:2004 are intended to ensure that comparable and reproducible results are obtained in different laboratories.ISO 22174:2004 has been established for food-borne pathogens in or isolated from food and feed matrices, but is also applicable to other matrices (e.g. environmental samples) and for the detection of non-pathogenic microorganisms.