I.S. CEN/TR 15214-2:2006
Current
The latest, up-to-date edition.
CHARACTERIZATION OF SLUDGES - DETECTION AND ENUMERATION OF ESCHERICHIA COLI IN SLUDGES, SOILS, SOIL IMPROVERS, GROWING MEDIA AND BIOWASTES - PART 2: MINIATURISED METHOD (MOST PROBABLE NUMBER) BY INOCULATION IN LIQUID MEDIUM
Hardcopy , PDF
English
01-01-2006
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
6 Sampling and hazards
6.1 Introduction
6.2 General
6.3 Storage
6.4 Handling
7 Reagents, diluents and culture media
7.1 Tryptone salt diluent
7.2 Special diluent (SD)
7.3 Demineralized or distilled water
7.4 Culture medium: MUG/EC medium
8 Procedure
8.1 Sample preparation
8.2 Analysis
9 Expression of results
9.1 Determination of the characteristic number
9.2 Calculation of the MPN and its confidence interval
10 Test report
11 Performance data
Annex A (informative) MPN Statistical Table
6 dilutions (1/20 to 1/2000000)/16 wells seeded per dilution
Annex B (informative) Synthetic sea salt
Annex C (informative) Performance data of the method
Annex D (normative) Quality criteria for the manufacturing of
the medium in microtitre plates (E.coli)
Bibliography
Specifies a miniaturized most probable number (MPN) method for the quantitative detection of Escherichia coli in sludges, soils, soil improvers, growing media and biowastes. It is suitable to evaluate the log reduction of E. coli through treatment as well as the quality of the end product.
| DocumentType |
Standard
|
| Pages |
30
|
| PublisherName |
National Standards Authority of Ireland
|
| Status |
Current
|
| Standards | Relationship |
| CEN/TR 15214-2:2006 | Identical |
| ISO 5667-13:2011 | Water quality Sampling Part 13: Guidance on sampling of sludges |
| EN 12880:2000 | Characterization of sludges - Determination of dry residue and water content |
| ISO 8199:2005 | Water quality General guidance on the enumeration of micro-organisms by culture |
| EN ISO 5667-13:2011 | Water quality - Sampling - Part 13: Guidance on sampling of sludges (ISO 5667-13:2011) |
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