Describes an ultraviolet (UV) fluorescence test method for the determination of the sulfur content of motor gasolines containing up to 3,7% (m/m) oxygen [including those blended with ethanol up to about 10% (V/V)], and of diesel fuels, including those containing up to about 10% (V/V) fatty acid methylester (FAME), having sulfur contents in the range 3 mg/kg to 500 mg/kg.