ISO 13322-2:2021

This document describes a method to transfer the images from particles having relative motion to binary images within practical systems, in which the particles in the images are individually separated. Images of moving particles are created by an optical image capture device. Effects of particle movement on the images are either minimized by the instrumentation or corrected by software procedures. This method is applicable to the particle images that are clearly distinguishable from static background. Further processing of the binary image, which is then considered as static, is described in ISO13322-1. A dynamic image analysis system is capable of measuring a higher number of particles compared to static image analysis systems. This document provides guidance on instrument qualification for particle size distribution measurements by using particulate reference materials. This document addresses the relative movement of the particles with respect to each other, the effect of particle movement on the image (motion blur), the movement and position along the optical axis (depth of field), and the orientation of the particles with respect to the camera.