ISO 17601:2016
Current
The latest, up-to-date edition.
Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil
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French, English
12-01-2016
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ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.
It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.
| Committee |
ISO/TC 190/SC 4
|
| DevelopmentNote |
Supersedes ISO/DIS 17601. (01/2016)
|
| DocumentType |
Standard
|
| Pages |
32
|
| PublisherName |
International Organization for Standardization
|
| Status |
Current
|
| Standards | Relationship |
| SS-EN ISO 17601 : 2018 | Identical |
| EN ISO 17601:2018 | Identical |
| UNI EN ISO 17601 : 2018 | Identical |
| SN EN ISO 17601:2018 | Identical |
| UNE-EN ISO 17601:2018 | Identical |
| I.S. EN ISO 17601:2018 | Identical |
| NEN EN ISO 17601 : 2018 | Identical |
| NF EN ISO 17601 : 2018 | Identical |
| NS EN ISO 17601 : 2018 | Identical |
| BS EN ISO 17601:2018 | Identical |
| NF ISO 17601 : 2016 | Identical |
| 17/30352199 DC : 0 | BS ISO 15799 - SOIL QUALITY - GUIDANCE ON THE ECOTOXICOLOGICAL CHARACTERIZATION OF SOILS AND SOIL MATERIALS |
| ISO 19204:2017 | Soil quality — Procedure for site-specific ecological risk assessment of soil contamination (soil quality TRIAD approach) |
| BS ISO 19204:2017 | Soil quality. Procedure for site-specific ecological risk assessment of soil contamination (soil quality TRIAD approach) |
| DIN ISO 19204:2015-09 (Draft) | SOIL QUALITY - PROCEDURE FOR SITE-SPECIFIC ECOLOGICAL RISK ASSESSMENT OF SOIL CONTAMINATION (SOIL QUALITY TRIAD APPROACH) (ISO 19204:2017) |
| ISO 10381-6:2009 | Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory |
| ISO 11063:2012 | Soil quality Method to directly extract DNA from soil samples |
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