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ISO 17601:2016

Withdrawn

Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

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Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

Available format(s)

Hardcopy , PDF

Language(s)

French, English

Published date

12-01-2016

Withdrawn date

02-11-2025

Superseded by

ISO 17601:2025

€65.00
Excluding VAT
Summarise this standard

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.

It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

Committee
ISO/TC 190/SC 4
DevelopmentNote
Supersedes ISO/DIS 17601. (01/2016)
DocumentType
Standard
Pages
32
PublisherName
International Organization for Standardization
Status
Withdrawn
SupersededBy
UnderRevision

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A newer version of this standard is available.

€65.00
Excluding VAT