ISO 23443:2020
Current
The latest, up-to-date edition.
Infant formula and adult nutritionals Determination of -carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)
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English
02-07-2020
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This document specifies a method for the quantitative determination of -carotene and lycopene in infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) and UV-visible detection. The application range runs from 1 g/100 g to 1 500 g/100 g for lycopene and from 1 g/100 g to 2 250 g/100 g for -carotene. Based on the single-laboratory validation, the limit of detection (LOD) was 0,1 g/100 g and the limit of quantification (LOQ) was 0,3 g/100 g for each carotenoid.
The method does not apply to materials that contain measurable levels of -apo-8-carotenal. The reproducibility data meets the requirements given in References [8] and [10].
Annex C specifies the determination of lutein. The reproducibility data does not meet the requirements given in Reference [9].
| DocumentType |
Standard
|
| Pages |
32
|
| PublisherName |
International Organization for Standardization
|
| Status |
Current
|
| Standards | Relationship |
| NEN-ISO 23443:2020 | Identical |
| BS ISO 23443:2020 | Identical |
| IS 17671 : 2021 | Identical |
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