ISO/TS 19006:2016
Current
The latest, up-to-date edition.
Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
Hardcopy , PDF
English
18-07-2016
ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.
The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.
| Committee |
ISO/TC 229
|
| DocumentType |
Technical Specification
|
| Pages |
21
|
| PublisherName |
International Organization for Standardization
|
| Status |
Current
|
| Standards | Relationship |
| NEN NVN ISO/TS 19006 : 2016 | Identical |
| PD ISO/TS 19006:2016 | Identical |
| ISO 29701:2010 | Nanotechnologies — Endotoxin test on nanomaterial samples for in vitro systems — Limulus amebocyte lysate (LAL) test |
| ISO/TS 18827:2017 | Nanotechnologies — Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials |
| ISO/TR 13097:2013 | Guidelines for the characterization of dispersion stability |
| ISO/TS 80004-2:2015 | Nanotechnologies — Vocabulary — Part 2: Nano-objects |
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