NEN EN 13704 : 2002

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Requirements
5 Test method
   5.1 Principle
   5.2 Materials and reagents
   5.3 Apparatus and glassware
   5.4 Preparation of spore test suspension and
        test solutions
   5.5 Procedure
   5.6 Calculation and expression of results
   5.7 Conclusion
   5.8 Test report
Annex A (normative) Preparation of Bacillus spore
        stock suspensions
   A.1 Material and reagents
   A.2 Preparation of Bacillus spore stock suspensions
Annex B (normative) Validation of dilution-neutralization
        and membrane filtration methods
   B.1 Principle
   B.2 Preparation of spore suspension
   B.3 Preparation of product test solution
   B.4 Test for validation
   B.5 Validation
Annex C (informative) Preparation of Clostridium
        sporogenes spore stock suspension
   C.1 Culture media and reagents
   C.2 Apparatus and glassware
   C.3 Preparation of regenerated media and incubation
        conditions
   C.4 Preparation of Clostridium spore stock suspension
Annex D (informative) Neutralizers
Annex E (informative) Rinsing liquids
Annex F (informative) Example of a typical test report
Annex G (informative) Referenced strains in national
        collections
   G.1 Bacillus subtilis
   G.2 Bacillus cereus
   G.3 Clostridium sporogenes
Annex H (informative) Information on the application and
        interpretation of European standards on chemical
        disinfectants and antiseptics
   H.1 General guidelines for the application and
        interpretation of test methods in accordance
        with European Standards for chemical disinfectants
        and antiseptics
   H.2 Guide to interpretation of tests for chemical
        disinfectants and antiseptics

Defines a test method (phase 2/step 1) and the minimum requirements for sporicidal activity of chemical disinfectant products that form a homogeneous, physically stable preparation in hard water and that are used in food, industrial, domestic and institutional areas, excluding areas and situations where disinfection is medically indicated and excluding products used on living tissues except those for hand hygiene in the above considered areas.