NEN EN 14349 : 2012

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Requirements
5 Test method
  5.1 Principle
  5.2 Materials and reagents
      5.2.1 Test organisms
      5.2.2 Culture media and reagents
      5.2.3 Test surface
  5.3 Apparatus and glassware
      5.3.1 General
      5.3.2 Usual microbiological laboratory equipment and, in
            particular, the following:
  5.4 Preparation of test organism suspensions and product test
      solutions
      5.4.1 Test organism suspensions
      5.4.2 Microbial test suspension
      5.4.3 Product test solutions
  5.5 Procedure for assessing the bactericidal activity of the
      product
      5.5.1 Experimental conditions (obligatory and additional)
      5.5.2 Test procedure
  5.6 Calculation and expression of results
      5.6.1 Calculation of viable counts (cfu/ml)
      5.6.2 Verification of methodology
      5.6.3 Expression of results
  5.7 Conclusion
  5.8 Test report
Annex A (normative) Validation of dilution neutralization
      A.1 Principle
      A.2 Preparation of microbial suspension and test surface
      A.3 Preparation of product test solution
      A.4 Test for validation
          A.4.1 General
          A.4.2 Procedure
          A.4.3 Counting of the neutralizer toxicity and dilution
                neutralization controls
      A.5 Validation
Annex B (informative) Suitable Neutralizers
Annex C (informative) Example of a typical test report
Annex D (informative) Referenced strains in National Collections
Annex E (informative) Information on the application and
        interpretation of European Standards on chemical
        disinfectants and antiseptics
      E.1 General guidelines for the application and
          interpretation of test methods in accordance with
          European Standards for chemical disinfectants and
          antiseptics:
      E.2 Guide to interpretation of tests for chemical
          disinfectants and antiseptics
Bibliography

Defines a test method and the minimum requirements for bactericidal activity of chemical disinfectant and antiseptic products that form a homogeneous physically stable preparation when diluted with hard water, or in the case of ready-to-use-products with water.