NS EN ISO 21570 : 2005 AMD 1 2013

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 General
   4.2 Amplification, detection and confirmation of
       PCR products
   4.3 Quantitation of PCR products
5 Reagents
6 Apparatus and equipment
7 Guidelines concerning the procedure
   7.1 General
   7.2 Target sequence stability
   7.3 Calibration of the analysis
   7.4 Quantitation considerations
   7.5 Quality assurance requirements
8 Interpretation
9 Expression of results
10 Test report
Annex A (informative) Target taxon-specific methods
        A.1 Target taxon-specific method for the
            absolute quantitation of the adh1 gene
            DNA of maize using real-time PCR
Annex B (informative) Screening methods
        B.1 Screening method for the relative
            quantitation of the 35S-promoter DNA of
            soya bean line GTS 40-3-2 using real-time
            PCR
Annex C (informative) Construct-specific methods
        C.1 Construct-specific method for the
            quantitation of soya bean line
            GTS 40-3-2 using real-time PCR (Methode 1)
        C.2 Construct-specific method for the
            quantitation of soya bean line
            GTS 40-3-2 using real-time PCR (Methode 2)
        C.3 Construct-specific method for the
            quantitation of Events176 maize DNA using
            real-time PCR
        C.4 Construct-specific method for the
            quantitation of soya bean line
            GTS 40-3-2 using real-time PCR
        C.5 Construct-specific method for the
            quantitation of maize line MON 810
            DNA using real-time PCR
        C.6 Construct-specific method for the
            quantitation of Events176 maize DNA using
            real-time PCR
        C.7 Construct-specific method for the
            quantitation of maize line Bt11
            DNA using real-time PCR
        C.8 Construct-specific method for the
            quantitation of maize line GA21 DNA
            using real-time PCR
        C.9 Construct-specific method for the
            quantitation of maize line T25 DNA
            using real-time PCR
Annex D (informative) Event-specific methods
        D.1 Event-specific method for the absolute
            and relative quantitation of maize line
            Bt11 DNA based on real-time PCR
        D.2 Event-specific method for the relative
            quantitation of maize line MON 810 DNA
            using real-time PCR
Bibliography

Describes the overall framework of quantitative methods for the detection of genetically modified organisms (GMOs) in foodstuffs, using the polymerase chain reaction (PCR).