PD CEN/TS 15634-2:2012
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
View Superseded by
Foodstuffs. Detection of food allergens by molecular biological methods Celery (Apium graveolens). Qualitative determination of a specific DNA sequence in cooked sausages by real-time PCR
Hardcopy , PDF
11-10-2019
English
29-02-2012
Important note : Users cannot be edited or removed once added to your Multi user PDF.
Foreword
1 Scope
2 Principle
3 Reagents
4 Apparatus and equipment
5 Analysis steps
6 Validation status and performance criteria
7 Sample type and amounts
8 Limit of detection
9 Interferences
10 Test report
11 Method performance studies
Bibliography
Defines a method for the qualitative detection of celery (Apium graveolens) in emulsion-type sausages (e.g. Frankfurter, Wiener).
| Committee |
AW/275
|
| DocumentType |
Standard
|
| Pages |
22
|
| PublisherName |
British Standards Institution
|
| Status |
Superseded
|
| SupersededBy |
This Technical Specification specifies a method for the qualitative detection of celery (Apium graveolens) in emulsion-type sausages (e.g. Frankfurter, Wiener).
Real-time PCR detection of celery is based on an 101 bp (base pair) sequence from the gene of the mannitol dehydrogenase (GenBank Acc. No. AF067082) of celery (Apium graveolens).
The method has been validated on emulsion-type sausages (Bavarian “Leberkäse??) spiked with celery. For this purpose meat batter containing mass fractions of 50 % pork meat, 25 % pork fat, 23 % crushed ice and 1,8 % of a mixture of sodium chloride, nitrite, nitrate, phosphates and ascorbates was prepared according to a standard procedure for emulsion-type sausage. The meat batter was spiked with either ground celery seeds or celery root powder to 1000 mg/kg. Lower spiking levels were obtained by diluting with celery-free meat batter. The batter was stuffed into casings and heated at 65 °C for 60 min [2].
| Standards | Relationship |
| CEN/TS 15634-2:2012 | Identical |
| ISO 24276:2006 | Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions |
| EN 15634-1:2009 | Foodstuffs - Detection of food allergens by molecular biological methods - Part 1: General considerations |
Access your standards online with a subscription
Features
-
Simple online access to standards, technical information and regulations.
-
Critical updates of standards and customisable alerts and notifications.
-
Multi-user online standards collection: secure, flexible and cost effective.